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The Panama Canal, Pearl Islands, and Darién Jungle: Tropical Wonders and Vibrant Cultures

<p>Embark on our only Central American expedition that travels solely through Panama, connecting you more deeply with the region's wildlife, culture, and famed modern engineering marvel. Aboard the National Geographic Quest, transit the historic Panama Canal by day and by night. Learn about the country&rsquo;s indigenous and colonial history while visiting local communities and two UNESCO World Heritage sites. Encounter monkeys, iguanas, tropical birds, and more as you venture into lush rainforests, a wildlife refuge, and an important national park. In the surrounding turquoise waters, snorkel pristine coral reefs which shelter an array of colorful marine life.&nbsp;</p>

Optional extras & upgrades

Optional excursions available. Please get in touch for more information.
07 December, 2025 to 14 December, 2025
More Cabin Categories & Prices Available
Please contact us for more information
Category 1. From $10,556 AUD pp
Cat 1 #301-306 Cabins feature two lower single beds that can convert to a Queen, a writing desk and two portholes. Each cabin has individual climate controls, allowing you to adjust the temperature to your liking. Botanically-inspired shampoo, shower gel, and lotion are all available in cabin bathrooms, as well as an Expedition Essential Kit. Hair dryers are also available in each cabin.
view cabin photo
14 December, 2025 to 21 December, 2025
More Cabin Categories & Prices Available
Please contact us for more information
Category 1. From $10,556 AUD pp
Cat 1 #301-306 Cabins feature two lower single beds that can convert to a Queen, a writing desk and two portholes. Each cabin has individual climate controls, allowing you to adjust the temperature to your liking. Botanically-inspired shampoo, shower gel, and lotion are all available in cabin bathrooms, as well as an Expedition Essential Kit. Hair dryers are also available in each cabin.
view cabin photo
09 January, 2026 to 16 January, 2026
More Cabin Categories & Prices Available
Please contact us for more information
Category 1. From $10,984 AUD pp
Cat 1 #301-306 Cabins feature two lower single beds that can convert to a Queen, a writing desk and two portholes. Each cabin has individual climate controls, allowing you to adjust the temperature to your liking. Botanically-inspired shampoo, shower gel, and lotion are all available in cabin bathrooms, as well as an Expedition Essential Kit. Hair dryers are also available in each cabin.
view cabin photo
16 January, 2026 to 23 January, 2026
More Cabin Categories & Prices Available
Please contact us for more information
Category 1. From $10,984 AUD pp
Cat 1 #301-306 Cabins feature two lower single beds that can convert to a Queen, a writing desk and two portholes. Each cabin has individual climate controls, allowing you to adjust the temperature to your liking. Botanically-inspired shampoo, shower gel, and lotion are all available in cabin bathrooms, as well as an Expedition Essential Kit. Hair dryers are also available in each cabin.
view cabin photo
06 December, 2026 to 13 December, 2026
More Cabin Categories & Prices Available
Please contact us for more information
Category 1. From $10,760 AUD pp
Cat 1 #301-306 Cabins feature two lower single beds that can convert to a Queen, a writing desk and two portholes. Each cabin has individual climate controls, allowing you to adjust the temperature to your liking. Botanically-inspired shampoo, shower gel, and lotion are all available in cabin bathrooms, as well as an Expedition Essential Kit. Hair dryers are also available in each cabin.
view cabin photo
13 December, 2026 to 20 December, 2026
More Cabin Categories & Prices Available
Please contact us for more information
Category 1. From $10,760 AUD pp
Cat 1 #301-306 Cabins feature two lower single beds that can convert to a Queen, a writing desk and two portholes. Each cabin has individual climate controls, allowing you to adjust the temperature to your liking. Botanically-inspired shampoo, shower gel, and lotion are all available in cabin bathrooms, as well as an Expedition Essential Kit. Hair dryers are also available in each cabin.
view cabin photo
29 January, 2027 to 05 February, 2027
More Cabin Categories & Prices Available
Please contact us for more information
Category 1. From $11,087 AUD pp
Cat 1 #301-306 Cabins feature two lower single beds that can convert to a Queen, a writing desk and two portholes. Each cabin has individual climate controls, allowing you to adjust the temperature to your liking. Botanically-inspired shampoo, shower gel, and lotion are all available in cabin bathrooms, as well as an Expedition Essential Kit. Hair dryers are also available in each cabin.
view cabin photo

The Panama Canal, Pearl Islands, and Darién Jungle: Tropical Wonders and Vibrant Cultures itinerary:

show reverse itinerary
DAY 1 Panama City, Panama / Colón / Embark Ship


Upon arrival in Panama City this afternoon, transfer by land to Colón and embark the National Geographic Quest. Meet your expedition staff and enjoy a traditional Congo music and dance performance. Marvel at the coastline ahead as we sail to the historic port of Portobelo.

Arrival Time: Arrive by 12:00 p.m. 
Arrival City: Panama City, Panama 

Meals Included: Dinner

DAY 2 Portobelo / Panama Canal Transit


This morning, explore the area on kayaks, stand-up paddleboards, Zodiac cruises upriver, or go for a snorkel. Portobelo is known for its Spanish colonial military architecture, which is designated collectively as a UNESCO World Heritage site. Visit several of the fortifications as well as the old Customs House, and hear the tales of Portobelo’s rich pirate history. Later on, set sail for the Panama Canal. As cargo ships tend to pass through the canal during the day, we’ll most likely begin our crossing at night, when the canal is dramatically lit. By special arrangement, National Geographic Quest will anchor in Gatún Lake near the Barro Colorado Nature Monument overnight.  

Meals Included: Breakfast, Lunch, Dinner

DAY 3 Gatún Lake / Panama Canal Transit


Today, choose hikes and Zodiac cruises at the Smithsonian Tropical Research Institute facility on Barro Colorado Island, or take a boat ride to Gamboa, where the Chagres River meets the Panama Canal, and hike on the Panama Rainforest Discovery Center trails. This evening, continue through the complex lock system of the Canal and venture into the Gulf of Panama. 

Meals Included: Breakfast, Lunch, Dinner

DAY 4 Isla Iguana Wildlife Refuge / Pedasí


This morning, we arrive at Isla Iguana Wildlife Refuge. Once a US Army bombing range, this protected area is home to black and green iguanas, sea turtles, and several species of birds and tropical fish. There is time to enjoy the beach and snorkel. In the afternoon, visit the mainland for a Spanish heritage cultural experience in the town of Pedasí on the scenic Azuero Peninsula.

Meals Included: Breakfast, Lunch, Dinner

DAY 5 Playa Muerto, Darién National Park


Playa Muerto village is located in Darién National Park, the largest protected area in Central America and a UNESCO World Heritage site which contains an exceptional array of habitats, from sandy beaches and mangroves to dense tropical forests teeming with wildlife. It is also home to the indigenous Emberá and Wounaan tribes. We’ll be greeted by village leaders from the Emberá who will share their customs, traditions, and stories about their deep respect for their natural surroundings. Later on, enjoy short nature walks, go birding, or choose to join a longer hike into the rainforest in search of the elusive harpy eagle, one of the world’s most powerful eagles.

Meals Included: Breakfast, Lunch, Dinner

DAYS 6-7 Pearl Islands


Approximately 200 islands make up the idyllic Pearl Islands. Explore several of the islands in the archipelago, including Isla del Rey and Isla Pacheca, as you learn about the history of the native populations and eventually the Spaniards and African slaves who arrived for the pearl trade. Deep rainforests, lush rivers, white sandy beaches, and shallow reefs provide great opportunities for hiking, sea kayaking, stand-up paddleboarding, Zodiac river cruises, beach activities, and snorkeling.

Meals Included: Breakfast, Lunch, Dinner

DAY 8 Balboa / Disembark Ship / Panama City, Panama


After breakfast, disembark in Balboa and transfer to the airport in Panama City for your flight home.

Departure Time: Depart anytime after 2:30 p.m. 
Departure City: Panama City, Panama 

Meals Included: Breakfast

Please note:

Itineraries are subject to change.

The Panama Canal, Pearl Islands, and Darién Jungle: Tropical Wonders and Vibrant Cultures reverse itinerary:

show main itinerary
Please note: *

Itineraries are subject to change.

DAY 8 Balboa / Disembark Ship / Panama City, Panama *


After breakfast, disembark in Balboa and transfer to the airport in Panama City for your flight home.

Departure Time: Depart anytime after 2:30 p.m. 
Departure City: Panama City, Panama 

Meals Included: Breakfast

DAYS 6-7 Pearl Islands *


Approximately 200 islands make up the idyllic Pearl Islands. Explore several of the islands in the archipelago, including Isla del Rey and Isla Pacheca, as you learn about the history of the native populations and eventually the Spaniards and African slaves who arrived for the pearl trade. Deep rainforests, lush rivers, white sandy beaches, and shallow reefs provide great opportunities for hiking, sea kayaking, stand-up paddleboarding, Zodiac river cruises, beach activities, and snorkeling.

Meals Included: Breakfast, Lunch, Dinner

DAY 5 Playa Muerto, Darién National Park *


Playa Muerto village is located in Darién National Park, the largest protected area in Central America and a UNESCO World Heritage site which contains an exceptional array of habitats, from sandy beaches and mangroves to dense tropical forests teeming with wildlife. It is also home to the indigenous Emberá and Wounaan tribes. We’ll be greeted by village leaders from the Emberá who will share their customs, traditions, and stories about their deep respect for their natural surroundings. Later on, enjoy short nature walks, go birding, or choose to join a longer hike into the rainforest in search of the elusive harpy eagle, one of the world’s most powerful eagles.

Meals Included: Breakfast, Lunch, Dinner

DAY 4 Isla Iguana Wildlife Refuge / Pedasí *


This morning, we arrive at Isla Iguana Wildlife Refuge. Once a US Army bombing range, this protected area is home to black and green iguanas, sea turtles, and several species of birds and tropical fish. There is time to enjoy the beach and snorkel. In the afternoon, visit the mainland for a Spanish heritage cultural experience in the town of Pedasí on the scenic Azuero Peninsula.

Meals Included: Breakfast, Lunch, Dinner

DAY 3 Gatún Lake / Panama Canal Transit *


Today, choose hikes and Zodiac cruises at the Smithsonian Tropical Research Institute facility on Barro Colorado Island, or take a boat ride to Gamboa, where the Chagres River meets the Panama Canal, and hike on the Panama Rainforest Discovery Center trails. This evening, continue through the complex lock system of the Canal and venture into the Gulf of Panama. 

Meals Included: Breakfast, Lunch, Dinner

DAY 2 Portobelo / Panama Canal Transit *


This morning, explore the area on kayaks, stand-up paddleboards, Zodiac cruises upriver, or go for a snorkel. Portobelo is known for its Spanish colonial military architecture, which is designated collectively as a UNESCO World Heritage site. Visit several of the fortifications as well as the old Customs House, and hear the tales of Portobelo’s rich pirate history. Later on, set sail for the Panama Canal. As cargo ships tend to pass through the canal during the day, we’ll most likely begin our crossing at night, when the canal is dramatically lit. By special arrangement, National Geographic Quest will anchor in Gatún Lake near the Barro Colorado Nature Monument overnight.  

Meals Included: Breakfast, Lunch, Dinner

DAY 1 Panama City, Panama / Colón / Embark Ship *


Upon arrival in Panama City this afternoon, transfer by land to Colón and embark the National Geographic Quest. Meet your expedition staff and enjoy a traditional Congo music and dance performance. Marvel at the coastline ahead as we sail to the historic port of Portobelo.

Arrival Time: Arrive by 12:00 p.m. 
Arrival City: Panama City, Panama 

Meals Included: Dinner

* = Indicative
Map for The Panama Canal, Pearl Islands, and Darién Jungle: Tropical Wonders and Vibrant Cultures
National Geographic Quest, the ship servicing The Panama Canal, Pearl Islands, and Darién Jungle: Tropical Wonders and Vibrant Cultures

National Geographic Quest

Vessel Type: Expedition Ship

Length: 72 metres

Passenger Capacity: 100 guests (in 50 cabins)

Built: 2017

Designed with decades of expedition experience in the region, National Geographic Quest more than comfortably accommodates 100 guests in 50 cabins. Her twin expedition craft landing platform allow us to rapidly get on and off the ship to take advantage of wildlife sightings and to ensure we maximize our time off the ship exploring. Created with a shallow draft, Quest draws only nine feet of water allowing her to sail into places where much larger ships cannot go.

PUBLIC AREAS: Global gallery; fitness centre; LEXspa; lounge with full service bar and facilities for films and presentations; observation deck; mudroom with lockers for expedition gear, and a partially covered sundeck with chairs and tables. Our ‘open bridge’ provides guests an opportunity to meet our officers and captain and learn about navigation.

MEALS: Served in single seatings with unassigned tables for an informal atmosphere and easy mingling. Breakfast is a wide selection buffet and dinner is served family-style. Menu emphasises local fare.

CABINS: All cabins face outside with windows or portholes, private facilities and climate controls. Some cabins have balconies.

EXPEDITION EQUIPMENT: Expedition landing craft, a fleet of 24 kayaks, Remotely Operated Vehicle (ROV), hydrophone, splashcam, underwater video camera, video microscope, snorkelling gear for all guests (where applicable).

SPECIAL FEATURES: Guest internet access, elevator, National Geographic certified photo instructor, a video chronicler and undersea specialist.

WELLNESS: The vessel is staffed by a Wellness Specialist and features a gym with an elliptical machine, treadmill, exercycles, handweights and resistance bands. Treatments in the LEXspa are available by appointment.

Cabin layout for National Geographic Quest
• Transit the legendary Panama Canal—plus enjoy an exclusive stop at the Smithsonian Tropical Research Institute’s Barro Colorado Island

• Discover the storied Spanish architecture and fascinating pirate history of Portobelo, a UNESCO World Heritage site

• Explore Isla Iguana Wildlife Refuge and spot sea turtles and black and green iguanas on the beach or while snorkeling offshore

• Meet the indigenous Emberá people of Darién in Playa Muerto Village and learn about their customs, traditions, and deep reverence for their natural surroundings

• Sea kayak, hike, Zodiac cruise, and snorkel in the tranquil Pearl Islands, a network of 200 islands known for turquoise waters, white-sand beaches, and an enduring connection to the pearl trade